Relation between basal sperm morphology and sperm recovered by swim up
DOI:
https://doi.org/10.26820/recimundo/2.(3).julio.2018.111-123Palabras clave:
Sperm morphology, sperm preparation, semen analysis, swim up technique.Resumen
Objective: The purpose of this study is to investigate a possible correlation between basal sperm morphology and sperm recovered by swim up technique, in patients with fertility problems. Methods: Semen analysis was performed in 112 patients following the laboratory manual for the examination and processing of human semen of the World Health Organization (WHO), fifth edition. Sperm were separated by swim up technique and sperm parameters were reevaluated. Relation between basal sperm morphology and sperm parameters after swim up were evaluated by correlation coefficient analysis. Results: Means of basal parameters were above lower limits established by the WHO. After swim up, the average of sperm recovery, total motility and progressive motility were 8.89 ± 0.86, 91.74 ± 0.97, 97.41 ± 0.66 respectively. Sperm parameters after swim up showed an apparently lower concentration of sperm recovered when normal sperm morphology is lower in basal samples; however, these results were not statistically significant. Conclusion: There is no correlation between basal sperm morphology and quality sperm recovered after swim up, despite an apparently lower concentration of sperm after sperm separation is observed in patients with a decreased normal sperm morphology.Descargas
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Avellino, G., Theva, D., & Oates, R. D. (2017). Common urologic diseases in older men and their treatment: how they impact fertility. Fertil Steril, 107(2), 305-311. doi:10.1016/j.fertnstert.2016.12.008
Badawy, A., Elnashar, A., & Eltotongy, M. (2009). Effect of sperm morphology and number on success of intrauterine insemination. Fertil Steril, 91(3), 777-781. doi:10.1016/j.fertnstert.2007.12.010
Chang, V., Garcia, A., Hitschfeld, N., & Härtel, S. (2017). Gold-standard for computer-assisted morphological sperm analysis. Comput Biol Med, 83, 143-150. doi:10.1016/j.compbiomed.2017.03.004
Chang, V., Heutte, L., Petitjean, C., Härtel, S., & Hitschfeld, N. (2017). Automatic classification of human sperm head morphology. Comput Biol Med, 84, 205-216. doi:10.1016/j.compbiomed.2017.03.029
Correa-Pérez, J., Fernández-Pelegrina, R., Córdova-Claudio, M., Kaskar, K., & Panayiotis, Z. (2003). Improvement of morphological deficiencies according to the degree of teratozoospermia: Is it possible to reach a comparable to normozoospermic semen specimen? Paper presented at the ASRM, United State.
Eisenberg, M. L., & Meldrum, D. (2017). Effects of age on fertility and sexual function. Fertil Steril, 107(2), 301-304. doi:10.1016/j.fertnstert.2016.12.018
Elberger, L., Grünwaldt, M. M., ivita, A. D., Arenas, G., Buxeda, P. S., & Fried, E. P. d. (2006). Sperm morphology before and afert capacitation. Paper presented at the ASRM, United States.
Erdem, A., Erdem, M., Gumuslu, S., Kulak, D., Oktem, M., & Karabacak., O. (2009). Sperm morphology before and after sperm preparation do not predict pregnancy in intrauterine insemination (IUI) cycles with husband sperm. Paper presented at the ASRM, United States.
Gatimel, N., Moreau, J., Parinaud, J., & Léandri, R. D. (2017). Sperm morphology: assessment, pathophysiology, clinical relevance, and state of the art in 2017. Andrology, 5(5), 845-862. doi:10.1111/andr.12389
Khandwala, Y. S., Zhang, C. A., Li, S., Behr, B., Guo, D., & Eisenberg, M. L. (2017). Racial Variation in Semen Quality at Fertility Evaluation. Urology, 106, 96-102. doi:10.1016/j.urology.2017.03.064
Kim, S. K., Jee, B. C., & Kim, S. H. (2015). Histone methylation and acetylation in ejaculated human sperm: effects of swim-up and smoking. Fertil Steril, 103(6), 1425-1431. doi:10.1016/j.fertnstert.2015.03.007
Lampiao, F., & du Plessis, S. S. (2006). Comparing the Multi-ZSC one-step standardized swim-up method to the double-wash swim-up method with regard to the effects of sperm separation on morphology, head morphometry, and acrosome reaction inducibility. Fertil Steril, 86(3), 739-741. doi:10.1016/j.fertnstert.2006.02.084
Mascarenhas, M. N., Flaxman, S. R., Boerma, T., Vanderpoel, S., & Stevens, G. A. (2012). National, regional, and global trends in infertility prevalence since 1990: a systematic analysis of 277 health surveys. PLoS Med, 9(12), e1001356. doi:10.1371/journal.pmed.1001356
Morshedi, M., Duran, H. E., Taylor, S., & Oehninger, S. (2003). Efficacy and pregnancy outcome of two methods of semen preparation for intrauterine insemination: a prospective randomized study. Fertil Steril, 79 Suppl 3, 1625-1632.
Murcia-Robayo, R. Y., Jouanisson, E., Beauchamp, G., & Diaw, M. (2018). Effects of staining method and clinician experience on the evaluation of stallion sperm morphology. Anim Reprod Sci, 188, 165-169. doi:10.1016/j.anireprosci.2017.11.021
Palini, S., Stefani, S., Primiterra, M., Benedetti, S., Barone, S., Carli, L., . . . Feichtinger, W. (2017). Comparison of in vitro fertilization outcomes in ICSI cycles after human sperm preparation by density gradient centrifugation and direct micro swim-up without centrifugation. JBRA Assist Reprod, 21(2), 89-93. doi:10.5935/1518-0557.20170022
Ramos, V. B., Cipriani, D. a. C., Araújo, E. S., Salvador, R. A., Senn, A. P., Frajblat, M., & Amaral, V. L. (2015). Sperm Selection Using Three Semen Processing Techniques. JBRA Assist Reprod, 19(4), 223-226. doi:10.5935/1518-0557.20150043
Rappa, K. L., Rodriguez, H. F., Hakkarainen, G. C., Anchan, R. M., Mutter, G. L., & Asghar, W. (2016). Sperm processing for advanced reproductive technologies: Where are we today? Biotechnol Adv, 34(5), 578-587. doi:10.1016/j.biotechadv.2016.01.007
Ricci, E., Al Beitawi, S., Cipriani, S., Candiani, M., Chiaffarino, F., Viganò, P., . . . Parazzini, F. (2017). Semen quality and alcohol intake: a systematic review and meta-analysis. Reprod Biomed Online, 34(1), 38-47. doi:10.1016/j.rbmo.2016.09.012
Ricci, G., Perticarari, S., Boscolo, R., Montico, M., Guaschino, S., & Presani, G. (2009). Semen preparation methods and sperm apoptosis: swim-up versus gradient-density centrifugation technique. Fertil Steril, 91(2), 632-638. doi:10.1016/j.fertnstert.2007.11.068
Sigman, M. (2017). Introduction: What to do with older prospective fathers: the risks of advanced paternal age. Fertil Steril, 107(2), 299-300. doi:10.1016/j.fertnstert.2016.12.020
Thijssen, A., Klerkx, E., Huyser, C., Bosmans, E., Campo, R., & Ombelet, W. (2014). Influence of temperature and sperm preparation on the quality of spermatozoa. Reprod Biomed Online, 28(4), 436-442. doi:10.1016/j.rbmo.2013.12.005
Urbano, L. F., Masson, P., VerMilyea, M., & Kam, M. (2017). Automatic Tracking and Motility Analysis of Human Sperm in Time-Lapse Images. IEEE Trans Med Imaging, 36(3), 792-801. doi:10.1109/TMI.2016.2630720
World Health Organization, D. o. R. H. a. R. (2010). WHO laboratory manual for the examination and procesing of human semen. In W. H. Organization (Series Ed.), W. H. Organization (Ed.), (pp. 287).
Yamanaka, M., Tomita, K., Hashimoto, S., Matsumoto, H., Satoh, M., Kato, H., . . . Morimoto, Y. (2016). Combination of density gradient centrifugation and swim-up methods effectively decreases morphologically abnormal sperms. J Reprod Dev, 62(6), 599-606. doi:10.1262/jrd.2016-112